Figure 4.

After neuronal activation in the rat hippocampus, the cytoplasmic HuR and HuR mRNA are progressively upregulated, whereas the cytoplasmic HuB and HuB mRNA are stably expressed. We administered 50 mg/kg of PTZ intraperitioneally. Rats were sacrificed at 2 and 4 h after the onset of the seizures. For each analysis, equal amounts of the cytoplasmic protein extracts or RNA isolated from the unstimulated (control) and PTZ-treated (2 and 4 h after the onset of seizures) rat hippocampi were used and analyzed by Western blotting or qRT-PCR, respectively. (A) HuR accumulates progressively in the hippocampal cell cytoplasm after PTZ-evoked neuronal activation. GAPDH was used as a loading control. Representative cropped Western blots are shown. Graph data is presented as fold change in the protein expression. One-way ANOVA was followed by Tukey's multiple comparisons test. Values are mean ± SEM (**p < 0.01; ***p < 0.001; n = 5). (B) HuB is stably expressed in the hippocampal cell cytoplasm after PTZ-evoked neuronal activation. GAPDH was used as a loading control. Representative cropped Western blots are shown. Graph data is presented as fold change in the protein expression. Kruskal–Wallis test was followed by Dunn's multiple comparisons test. Values are mean ± SEM (n = 5). (C) HuR mRNA accumulates progressively after PTZ-evoked neuronal activation in the rat hippocampus. HuR mRNA expression was normalized against 18S rRNA expression. Data are presented as fold change in mRNA expression. One-way ANOVA was followed by Tukey's multiple comparisons test. Values are mean ± SEM (*p < 0.05; **p < 0.01; ***p < 0.001; n = 5). (D) HuB mRNA is stably expressed after PTZ-evoked neuronal activation in the rat hippocampus. HuB mRNA expression was normalized against 18S rRNA expression. Data are presented as fold change in mRNA expression. Kruskal–Wallis test was followed by the Dunn's multiple comparisons test. Values are mean ± SEM (n = 5).