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. 2018 Apr 9;12:224. doi: 10.3389/fnins.2018.00224

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Copyright © 2018 Zybura-Broda, Wolder-Gontarek, Ambrozek-Latecka, Choros, Bogusz, Wilemska-Dziaduszycka and Rylski.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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HuR and HuB upregulate Mmp-9 mRNA expression in activated rat hippocampal neurons. (A) HuR overexpression increases Mmp-9 mRNA in bicuculline-treated rat hippocampal neuronal cultures. The cultures were transfected by the HuR overexpression construct HuR/pGW1 or the mock control vector pGW1 using the Neon Transfection System. On Day 7 in vitro (DIV 7), the cultures were treated with bicuculline. mRNA content was analyzed at 4 h after neuronal activation by qRT-PCR. Mmp-9 expression was normalized against 18S rRNA expression. Data are presented as fold change in mRNA expression. Two-tailed Student's t-test. Values are mean ± SEM (**p < 0.01; n = 5). (B) HuR silencing downregulates Mmp-9 mRNA in bicuculline-treated rat hippocampal neuronal cultures. The cultures were transfected by HuR siRNA or control siRNA using the Neon Transfection System. On Day 7 in vitro (DIV 7), the cultures were treated with bicuculline. mRNA content was analyzed 4 h after neuronal activation by qRT-PCR. Mmp-9 expression was normalized against 18S rRNA expression. Data are presented as fold change in mRNA expression. Mann–Whitney U-test. Values are mean ± SEM (*p < 0.05; n = 5). (C) HuB overexpression increases Mmp-9 mRNA in bicuculline-treated rat hippocampal neuronal cultures. The cultures were transfected by the HuB overexpression construct HuB/pGW1 or mock control vector pGW1 using the Neon Transfection System. On Day 7 in vitro (DIV 7), the cultures were treated with bicuculline. mRNA content was analyzed 4 h after neuronal activation by qRT-PCR. Mmp−9 expression was normalized against 18S rRNA expression. Data are presented as fold change in mRNA expression. Two-tailed Student's t-test. Values are mean ± SEM (*p < 0.05; n = 5). (D) HuB silencing downregulates Mmp-9 mRNA in bicuculline-treated rat hippocampal neuronal cultures. The cultures were transfected by the HuB silencing construct HuB/pSuper or mock control vector pSuper using the Neon Transfection System. On Day 7 in vitro (DIV 7), the cultures were treated with bicuculline. mRNA content was analyzed 4 h after neuronal activation by qRT-PCR. Mmp-9 expression was normalized against 18S rRNA expression. Data are presented as fold change in mRNA expression. Two-tailed Student's t-test. Values are mean ± SEM (*p < 0.05; n = 5).