Table 2.
Antibodies for WB, neutralization, and REMSA Supershift.
| Antibody | Supplier, catalog number | Methoda |
|---|---|---|
| HuB (N-15) | Santa Cruz Biotechnology, sc-5982 | WB (1:300), REMSA Supershift, neutralization |
| HuC (G-15) | Santa Cruz Biotechnology, sc-5981 | REMSA Supershift, neutralization |
| HuD (L-20) | Santa Cruz Biotechnology, sc-5979 | REMSA Supershift |
| HuR (3A2) | Santa Cruz Biotechnology, sc-5261 | WB (1:1,000), REMSA Supershift, neutralization |
| GAPDH | Millipore, MAB374 | WB (1:5,000) |
| TBP | Pierce (Thermo Scientific), MA5-14739 | WB (1:1,000) |
| normal mouse IgG | Santa Cruz Biotechnology, sc-2025 | REMSA Supershift, neutralization |
| normal goat IgG | Santa Cruz Biotechnology, sc-2028 | REMSA Supershift, neutralization |
a
In parentheses, dilutions used for WB are shown.
WB, Western Blot; REMSA, RNA electrophoretic mobility shift assay.