Table 3.
Oligonucleotides used in REMSA and REMSA Supershift experiments.
Oligonucleotide | Sequence |
---|---|
UTR-ARE-1 | 5′-CCCUUUUAUUUAUUAUGUAUG-3′ |
UTR-ARE-2 | 5′-ACAUGUAUUUAACCUAUAGAA-3′ |
UTR-ARE-3 | 5′-CAUCAAACAUUUAUUGUGAGC-3′ |
UTR-ARE-4 | 5′-CAGAGGAAUUUAUUGGAUGUU-3′ |
The oligonucleotides are derived from the region encompassing the AUUUA-rich motifs localized in the rat Mmp-9 mRNA 3′UTR. The consensus sequences are indicated in boldface.