Figure 2.

Phagosomes containing Cryptococcus neoformans acquire V-ATPase following phagocytosis that is lost prior to exocytosis. (A) Confocal time lapse microscopy of C. neoformans phagocytosis by wild type D. discoideum strain Ax2. Representative time lapse from three independent experiments. Images were captured every 10 s. VatM is a subunit of the D. discoideum V-ATPase complex. Arrow indicates phagocytosed cryptococcal cell. Inset box is a magnification of phagosome containing cryptococcal cell demonstrating acquisition of V-ATPase. (B) Confocal time lapse microscopy of C. neoformans exocytosis by wild type D. discoideum strain Ax2. Representative time lapse from three independent experiments. Images were captured every 5 s. Arrow indicates exocytosed cryptococcal cell. Inset box is a magnification of exocytosed cryptococcal cell demonstrating absence of V-ATPase prior to exocytosis. Scale bars 10 μm.