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. 2018 Apr 9;8:108. doi: 10.3389/fcimb.2018.00108

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Copyright © 2018 Watkins, Andrews, Wynn, Barisch, King and Johnston.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exocytosis and vomocytosis of C. neoformans cap59 and plb1 mutants are indistinguishable from wild type H99. (A) Quantification of the percentage of non-constitutive exocytosis of C. neoformans mutants from wild-type Ax2 D. discoideum treated with latrunculin A from three independent 12 h time lapses. Total of 45 phagosomes were analyzed for wild type H99, 90 for cap59 and 90 for plb1. P-values are Fisher's exact test. (B) Transit times of C. neoformans H99 wild type, mutant cap59 and plb1. Phagosomes analyzed the same as (A). P-values for between cryptococcal strain significance tests are non-significant in all cases. P-values are Mann-Whitney test.