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. 2018 Apr 9;9:307. doi: 10.3389/fphys.2018.00307

Figure 5.

Figure 5

Inhibition autophagy abrogated the protective effects of pravastatin (PS) in BM-EPCs under 24 h of dexamethasone (Dex) exposure. (A) Effect of autophagy on the cell viability and LDH release in BM-EPCs exposed to Dex stress. (1) Western blot analysis for the regulatory effect of Rapa (10 nM, autophagy inductor) and 3-MA (5 mM, autophagy inhibitor) on autophagy in BM- EPCs upon Dex (1 μM) stress for 24 h. (2) The cell viability was detected by a CCK-8 assay and 3) LDH release was measured by colorimetric assay. (B) 3-MA significantly decreased the viability rate in PS (1 μM)-treated BM-EPCs. (C) 3-MA significantly increased the LDH release in PS-treated BM-EPCs. (D,E) The protection of PS on BM-EPCs tube formation and migratory capacities were reversed by 3-MA under 24 h of Dex exposure. Values are expressed as the mean ± SEM of three independent experiments. *p < 0.05, **p < 0.01 vs. Control; #p < 0.05, ##p < 0.01 vs. Dex.