Table II.
Specificity constants and ΔΔG‡ values for high-pI α-glucosidase catalyzed hydrolysis of monodeoxy maltose analogs
| Substrate | Vmax/Km | ΔΔG‡ |
|---|---|---|
| kJ mol−1 | ||
| Methyl β-maltoside | 9.51 × 10−3 ± 1.77 × 10−3 | — |
| Methyl 1-deoxy-β-maltoside | 1.05 × 10−2 ± 1.02 × 10−3 | −0.3 |
| Methyl 1,2-dideoxy-β-maltoside | 1.17 × 10−2 ± 1.14 × 10−3 | −0.3 |
| Methyl 3-deoxy-β-maltoside | 6.73 × 10−4 ± 1.09 × 10−5 | 7.0 |
| 2′-Deoxy maltose | 2.83 × 10−4 ± 3.00 × 10−5 | 9.3 |
| 3′-Deoxy maltose | 1.66 × 10−4 ± 2.62 × 10−5 | 10.7 |
| 4′-Deoxy maltose | 7.53 × 10−6 ± 1.47 × 10−7 | 18.9 |
| 6′-Deoxy maltose | 8.09 × 10−6 ± 1.45 × 10−7 | 18.7 |
Assays were performed at 37°C in 50 mm sodium acetate, pH 4.5. ΔΔG‡ was calculated as −RTln[Vmax/Km)a/(Vmax/Km)b], where a and b designate analog and parent compound, respectively.