Fig. 3.

RV vascular rarefaction in Sugen5416 + Hypoxia- pulmonary hypertension (SuHx-PH) rats. A: immunofluorescence overlay of WGA (wheat germ agglutinin, green, cardiomyocytes), Lectin Griffonia simplicifolia (red, endothelial cells) and DAPI (blue, nuclei) stains in female normoxic control or SuHx-PH rats. B: capillaries were quantified from 4 fields per animal; n = 4 animals /group. Images were taken at ×20 magnification; scale bars, 50 µm. Data are expressed as means ± SE *P < 0.05 vs. normoxic control. Note that this analysis used traditional 2-D analysis similar to previous studies in the field.