Fig. 1.

Cigarette smoke disrupts the production of proinflammatory mediators in primary human small airway epithelial cells (SAEC) in response to viral mimetic polyinosinic polycytidylic acid (poly I:C). Primary human SAEC were exposed to either air or cigarette smoke for 60 min at the air-liquid interface. Following exposure, the basal medium was changed, and the apical surface was treated with indicated doses of poly I:C. A: interferon-γ-induced protein 10 (IP-10) levels from donor 1 treated with increasing doses of poly I:C. B: Alamar Blue dye reduction assay was performed on cells from donor 1 24 h after treatment with poly I:C. C–E: IP-10, IL-6, and IL-8 were measured in culture supernatants by ELISA from 3 different donors. Bars are means ± SD of triplicate cultures. ***P < 0.001 by 2-way ANOVA with Tukey post hoc correction.