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Cigarette smoke impairs epithelial cell response to influenza A virus (IAV). SAEC were exposed to cigarette smoke for 60 min and then infected with WT PR8 (MOI 2). IP-10 (A), IL-6 (B), and IL-8 (C) were measured in culture supernatants. D: air- or smoke-exposed SAEC were infected with Puerto Rico/08/1934 expressing mCherry (PR8 mCherry; MOI 0.01) and imaged 24 h postinfection.
