Figure 1.

Effects of culture media and genotype on maintenance of hippocampal structure in long-term OHSC. A: DAPI-positive nuclei (blue) according to DIV in cultured hippocampal slices of adult WT and 3xTg-AD mouse. Yellow arrows indicate DAPI positive regions. B: Quantification of the DAPI-positive fluorescent intensity according to media and genotype by time. Each point, mean±SE. Scale bars, 1 mm. ^**p<0.01 and ^***p<0.001 indicate significance when compared to SF-WT condition in Bonferroni post hoc analysis. DAPI: 4’,6-diamidino-2-phenylindole, WT: wild type, AD: Alzheimer’s disease (model), DIV: days in vitro, SF: serum-free, SC: serum-containing.