Figure 2.

Effects of age and genotype on maintenance of hippocampal structure in long-term OHSC. A: Time-dependent changes on intensity of DAPI-positive nuclei (blue) in cultured hippocampal slices from young AD, old WT, and old AD mouse with serum-free medium. Yellow arrows indicate DAPI positive regions. B: Quantification of the DAPI-positive fluorescent intensity according to DIV in the images. No significant difference among 3 groups. Each point, mean±SE. Scale bars, 1 mm. DAPI: 4’,6-diamidino-2-phenylindole, WT: wild type, AD: Alzheimer’s disease (model), DIV: days in vitro, SF: serum-free, SC: serum-containing.