Skip to main content
. 2000 May;123(1):319–326. doi: 10.1104/pp.123.1.319

Figure 2.

Figure 2

Activity gel assay for ADGase, PGM, and PGI in wild-type and mutant leaf extracts. Leaf extracts from the wild type (20 μL in lanes 1, 3, and 10; 10 μL in lane 7; and 0.4 μL in lane 5), TSY254 (20 μL in lanes 2, 4, and 8; 10 μL in lane 6), and TSY254/+ (20 μL in lane 9) were separated in a 7% (w/v) native PAGE and assayed for the ADGase, PGM, and PGI activity. ADGase activity was detected by calcium pyrophosphate precipitation; PGM and PGI activity were detected as the colored formazan formation in enzymatic coupling reactions. Arrowheads indicate the positions of the plastidial forms of the enzymes (Caspar et al., 1985).