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. 2018 Mar;13(3):541–548. doi: 10.4103/1673-5374.228760

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Copyright: © Neural Regeneration Research

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as the author is credited and the new creations are licensed under the identical terms.

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Real-time PCR for synaptophysin mRNA.

mRNA levels were assessed in SAM hippocampal tissues by real-time PCR. The products were quantified using β-actin as an internal reference. The relative level of mRNA was calculated as 2^-ΔΔCT. Data are expressed as the mean ± SD, and were analyzed by one-way analysis of variance followed by the Student-Newman-Keuls test. *P < 0.05, vs. RC; #P < 0.05, vs. PC; &P < 0.05, vs. PT; †P < 0.05, vs. PTA. RC: SAMR1 control group; PC: SAMP8 control group; PT: SAMP8 NSCs transplantation group; PTA: SAMP8 NSC transplantation with acupuncture group; PTD: SAMP8 NSC transplantation with HuangDiSan group; PTC: SAMP8 NSC transplantation with acupuncture and HuangDiSan combination group. PCR: polymerase chain reaction; SAM: senescence-accelerated mice; NSC: neural stem cell.