Figure 3.

Apoptotic changes in Müller cells (detected by Hoechst 33258 staining) treated with Y-27632 at 10 μM after 24 hours under oxidative stress, observed by fluorescence microscopy.

(A–C) Apoptosis in the control, CoCl₂ and CoCl₂ + Y27632 groups, respectively (×200). (D–F) Apoptosis in the control, H₂O₂ and H₂O₂ + Y27632 groups (× 200). Control group: apoptotic cells are rare. CoCl₂ and H₂O₂ groups: the number of apoptotic cells increases, and Y27632 mitigates CoCl₂- and H₂O₂-induced cell injury. Arrows indicate apoptotic cells. (G) Quantitation of retinal Müller cell apoptosis. Data are expressed as the mean ± SD and were analyzed by one-way analysis of variance followed by the least significant difference test. All experiments were performed in triplicate. ##P < 0.01, vs. control group.