Figure 4.

ROS generation in retinal Müller cells incubated with 10 μM Y-27632 for 24 hours, detected by DCFH-DA and observed by fluorescence microscopy (original magnification, 100×).

(A–C) ROS generation in the negative control, CoCl₂ and CoCl₂ + Y27632 groups, respectively; (D–F) ROS generation in the positive control, H₂O₂ and H₂O₂ + Y27632 groups, respectively. Control group: No obvious ROS generation; CoCl2 and H₂O₂ groups: ROS generation increases, and Y27632 inhibits ROS generation induced by CoCl₂ and H₂O₂. Arrows indicate ROS generated by Müller cells. ROS: Reactive oxygen species.