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. 2018 May;24(5):643–655. doi: 10.1261/rna.064881.117

FIGURE 3.

FIGURE 3.

Each miRNA gene produces many isomiRs with varying expression levels. (A) Contribution of the five most expressed isomiRs to the total miRNA expression levels in frontal cortex (FCx, accounting for 96.4% of all reads) and in hippocampus (Hpc, accounting for 96.0% of all reads). (B) The proportion of canonical sequences represented within the top five expressed isomiRs in Hpc (87.7%) and FCx (87.2%). (C) Distribution of the five most common isomiRs of miR-22 in the FCx. Top miR-22-3p isomiRs are expressed at a 1640-fold higher level than the 5p arm top isomiRs. The canonical isomiR is the most common, constituting 93.9% of the 3p arm expression. The canonical miRNA sequence is depicted by vertical lines. RNA-edited nucleotides are shown in red and 3′ addition in blue. (D) Distribution of the five most common isomiRs of miR-411 in Hpc. Top miR-411-5p isomiRs are more commonly expressed than miR-411-3p (50:1). Although the canonical isomiR is the most prevalent isomiR, it only constitutes 37.7% of the 5p expression. The second most common isomiR has an alternative 5′ start site and accounts for 36.2% of the overall miR-411-5p expression. (E) The expression levels of the second most common miR-411-5p isomiR vary across the six mouse strains (adjusted P = 0.036).