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(A) 2D NOESY and 1D spectrum of r(GACGAIUGUCA)2 (6I) at 1.1 mM strand. Temperature is −2°C with 50 msec mixing time. Blue, red, and green circles correspond to NOESY cross-peaks between protons indicated by colored arrows in Figure 7C and D. (B) Imino proton spectrum at 1°C of 6I at 1.1 mM strand (top) and diluted 30-fold (bottom, 15-fold vertical expansion). Peaks remaining at low concentration belong to a hairpin conformation.
