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. 2018 May;24(5):688–703. doi: 10.1261/rna.063313.117

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© 2018 Hsu et al.; Published by Cold Spring Harbor Laboratory Press for the RNA Society

This article is distributed exclusively by the RNA Society for the first 12 months after the full-issue publication date (see http://rnajournal.cshlp.org/site/misc/terms.xhtml). After 12 months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

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FIGURE 2. — AEG-1 associates with actively translating mRNAs. (A) Schematic of experimental protocol for examining AEG-1 RNA-binding activity by ultracentrifugation analysis. Cell lysates were ultracentrifuged subsequent to RNase or control digestion. The polysome fraction (ribosome pellet) was analyzed by immunoblot. (B) Immunoblot analysis of AEG-1, poly(A)-binding protein (PABP), and ribosomal protein (RPL17) in the pellet fraction with/without RNase digestion. (C) Schematic of experimental protocol for the analysis of mRNA-binding activity of AEG-1 by polyribosome profiling. (D) Immunoblot analysis of AEG-1 and ribosomal protein (RPL17) in sucrose density gradient fractions. (E) Mobility of AEG-1 in sucrose density gradient velocity sedimentation in control (−EDTA) or after polyribosome disassembly (+EDTA). Immunoblot analysis of the lysosomal membrane protein NPC1 is illustrated as a membrane protein control.