The AEG-1 RNA interactome is highly enriched in resident endomembrane protein- and transmembrane domain-encoding transcripts. (A) Venn diagram depicting common and unique mRNAs identified in the HITS-CLIP and PAR-CLIP studies. (B) Reproducibility of AEG-1 bound mRNAs, comparing HITS-CLIP duplicate libraries and HITS-CLIP vs. PAR-CLIP. (C) Subcellular locales of AEG-1 HITS-CLIP genes, depicting endomembrane organelle, cytosolic and secreted protein-encoding genes on the left. The composition of the category “endomembrane” is depicted on the right, including plasma membrane, endoplasmic reticulum, lysosome, Golgi apparatus, mitochondrion, and nuclear envelope. (D) Gene ontology analysis of AEG-1 associated mRNAs. The mRNAs were subjected to gene ontology analysis using DAVID (Huang et al. 2009a,b). Endomembrane organelle distributions of proteins encoded in the AEG-1 bound mRNAs are illustrated. (E) GO category enrichments of the AEG-1 RNA interactome in the categories “endomembrane proteins” and “cytosolic proteins.”