Figure 6.

Subcellular localization of short-chain ACOX in pumpkin cotyledons. A, An extract from 5-d-old dark-grown pumpkin cotyledons was fractionated by Suc density gradient centrifugation and fractions were tested for enzyme activity: ●, catalase (μmol min⁻¹ mL⁻¹ × 30); ▴, cytochrome c oxidase (nmol min⁻¹ mL⁻¹ × 4); ▵, long-chain ACOX (μmol min⁻¹ mL⁻¹); ○, short-chain ACOX (μmol min⁻¹ mL⁻¹); solid line, Suc concentration. Long-chain ACOX and short-chain ACOX were tested employing palmitoyl-CoA and hexanoyl-CoA, respectively. B, Immunological detection of pumpkin short-chain ACOX by the use of antibodies raised against Arabidopsis short-chain ACOX. Fractions corresponding to mitochondria and peroxisomes are indicated. The position of the prestained molecular mass standard (48.5 kD) is indicated on the right.