Table III.
Distribution of GLDH activity in subcellular fractions of potato leaves
| Subcellular Fraction | GLDH | NADPH-Cyt c Reductase | Cyt c Oxidase | Chlorophylls |
|---|---|---|---|---|
| μmol min−1 mg−1 protein | mg mg−1 protein | |||
| Chloroplasts | 1.6 ± 1.0 | 0.3 ± 0.3 | 0.16 ± 0.02 | 390 ± 10 |
| (2,200g pellet) | (4.5%) | (5.47%) | (6%) | (80.7%) |
| Mitochondrial- and peroxisomal-enriched | 25.3 ± 3.2 | 0 | 2.3 ± 0.7 | 83.5 ± 5 |
| fraction (14,000g pellet) | (71.2%) | (83.48%) | (17%) | |
| Microsomes | 8.4 ± 1.2 | 5.0 ± 0.4 | 0.2 ± 0.01 | 8.3 ± 0.5 |
| (100,000g pellet) | (23.6%) | (91.24%) | (7.26%) | (1.7%) |
| Cytosol | 0.25 ± 0.13 | 0.18 ± 0.15 | 0.09 ± 0.03 | 1.4 ± 0.5 |
| (100,000g supernatant) | (0.7%) | (3.28%) | (3.27%) | (3.5%) |
Values between parentheses indicate a percentage of enzyme activities. Values are means of three different experiments ± sd.