Table VI.
GLDH activity in mitochondrial inner- and outer-membrane enriched fractions from potato leaves
Fraction | CCO | A-CCR | GLDH | GLDH/CCO | GLDH/A-CCR |
---|---|---|---|---|---|
nmol Cyt c min−1 mg−1 protein | |||||
Inner-membrane enriched | 260 ± 22 | 143 ± 60 | 14 ± 4 | 0.054 ± 0.01 | 0.11 ± 0.01 |
Outer-membrane enriched | 108 ± 13 | 3100 ± 775 | 7 ± 3 | 0.065 ± 0.01 | 0.004 ± 0.001 |
Cyt c oxidase (CCO) and antimycin A-resistant Cyt c reductase (A-CCR) were used as inner and outer membrane markers, respectively. Values are means of three different experiments ± sd.