Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Mar 1;32(5-6):341–346. doi: 10.1101/gad.311639.118

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Zhang et al.; Published by Cold Spring Harbor Laboratory Press

This article is distributed exclusively by Cold Spring Harbor Laboratory Press for the first six months after the full-issue publication date (see http://genesdev.cshlp.org/site/misc/terms.xhtml). After six months, it is available under a Creative Commons License (Attribution-NonCommercial 4.0 International), as described at http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Figure 4. — Zinc enhances the binding ability of DOT1L^CC2 to AF10^OM-LZ. (A) Summary of the quantitative binding constants between various divalent metal ions and the DOT1L^CC2–AF10^OM-LZ complex. (B) There are two conserved zinc-binding sites in the DOT1L^CC2–AF10^OM-LZ complex. (C) Dissociation constants of zinc with DOT1L^CC2, AF10^OM-LZ, and the DOT1L^CC2–AF10^OM-LZ complex. Mutation of zinc-coordinating residues (AF10^H764A/E774A) abolishes the zinc binding. (D) ITC data show that zinc enhances the binding of DOT1L^CC2 to AF10^OM-LZ. (E) The thermal shift melting curves of the DOT1L^CC2–AF10^OM-LZ complex in the presence or absence of zinc measured by differential static light scattering.