Figure 2.

Conversion of 1 a (20 mm) into (S)‐2 a at various concentrations of HCOONa as final hydride donor. The bio‐transformations were performed in a biphasic system [KPi (pH 8, 50 mm)/n‐heptane (1:1, v/v, 1 mL total reaction volume)] containing E. coli/Fus‐SMO lyophilised cells (10 mg mL⁻¹), NAD⁺ (1 mm), FAD (50 μm), HCOONa (0–100 mm) and Cb‐FDH (10 μm). The mixtures were incubated at 30 °C, 180 rpm for 24 h. Levels of conversion are the averages of two independent sets of experiments, both in duplicate (for a complete dataset including standard deviations see Section S6). The enantiomeric excess was determined by chiral HPLC to be >99 % S.