Table 1.
Data collection and refinement statistics for crystals of apo- and sterol-bound Lam4S2
r.m.s., root mean square; n/a, not applicable.
| Apo-Lam4a | Lam4 (sterol-bound)a | |
|---|---|---|
| Data collection | ||
| Space group | I212121 | P1 |
| Cell dimensions | ||
| a, b, c (Å) | 45.6, 67.9, 132.0 | 38.2, 47.3, 56.0 |
| α, β, γ (°) | 90, 90, 90 | 111.2, 101.6, 100.3 |
| Wavelength (Å) | 0.97494 | 1.00001 |
| Resolution (Å) | 50–2.19 (2.28–2.19) | 50.0–2.20 (2.27–2.20) |
| Rsym | 0.132 (0.664) | 0.09 (0.259) |
| I/σI | 23.6 (4.8) | 17.8 (4.8) |
| Completeness (%) | 100 (100) | 98.3 (97.1) |
| Redundancy | 14.0 (12.8) | 3.9 (3.8) |
| Refinement | ||
| Resolution (Å) | 43.1–2.19 (2.27–2.19) | 42.0–2.20 (2.28–2.20) |
| No. reflections | 10,837 | 17,131 |
| Rwork/Rfree (%) | 0.186/0.248 | 0.166/0.230 |
| No. of atoms | ||
| Protein | 1601 | 3308 |
| Ligand | n/a | 58 |
| Water | 48 | 143 |
| B-factors (Å2) | 45.2 | 30.5 |
| Protein | 45.3 | 30.6 |
| Ligands | n/a | 26.0 |
| Water | 42.0 | 30.6 |
| r.m.s. deviations | ||
| Bond lengths (Å) | 0.008 | 0.008 |
| Bond angles (°) | 0.901 | 0.999 |
| Ramachandran plot | ||
| Favored (%) | 96.9 | 98 |
| Allowed (%) | 3.1 | 2 |
| Disallowed (%) | 0.0 | 0 |
a Values in parentheses correspond to the highest resolution shell.