Figure 3.

The N-terminal extension of Salvador SARAH enhances binding to Hippo. A pulldown experiment was performed to analyze the amount of binding of His₆-SUMO-tagged Salvador SARAH domain variants with (residues 530–608) or without (residues 552–608) the N-terminal extension to either blank resin or resin coupled to the Hippo SARAH domain and analyzed by Coomassie-stained SDS-PAGE. The binding experiment was performed seven times and a representative gel is shown (left). The ratio of bound Salvador to Hippo SARAH domains was calculated from band intensities and normalized between replicates (right). The p value between Salvador SARAH domain variants either with (530–608) or without the N-terminal extension (552–608) is ≤0.0001.