Table 3.
Antibodies, clone, dilution, and source
| Antibody | Clone | Dilution | Source | Catalog no. |
|---|---|---|---|---|
| Anti-HA (16B12) (DyLight 488) | Mouse | 1:500 | Abcam | Ab117488 |
| Goat anti-mouse IgG secondary antibody, Alexa Fluor 594 | Goat | 1:500 | Thermo Fisher Scientific | A-11032 |
| Ubiquitin (P4D1) Lys63/Lys48 mouse mAb | Mouse | 1:1000 | Cell Signaling | 3936S |
| Anti-HA.11 epitope tag antibody | Mouse | 1:1000 | Biolegend | 901503 |
| Monoclonal anti-β-actin antibody | Mouse | 1:5000 | Sigma | A1978 |
| β-arrestin 1/2 rabbit mAb | Rabbit | 1:1000 | Cell Signaling | 4674S |
| Anti-FLAG antibody | Rabbit | 1:1000 | Sigma | F7425 |
| Lys63 linkage-specific polyubiquitin rabbit mAb | Rabbit | 1:500 | Cell Signaling | 12930 |
| Phospho-p44/42 MAPK (ERK1/2) (Thr202/Tyr204) Rabbit mAb | Rabbit | 1:1000 | Cell Signaling | 9101S |
| p44/42 MAPK (ERK1/2) rabbit mAb | Rabbit | 1:1000 | Cell Signaling | 4695S |
| Phospho-p38 MAPK (Thr180/Tyr182) rabbit mAb | Rabbit | 1:1000 | Cell Signaling | 4511S |
| p38 MAPK antibody | Rabbit | 1:1000 | Cell Signaling | 9212S |
| Phospho-SAPK/JNK (Thr183/Tyr185) rabbit mAb | Rabbit | 1:1000 | Cell Signaling | 4668S |
| SAPK/JNK antibody | Rabbit | 1:1000 | Cell Signaling | 9252P |