Figure 4.

Cbp3 and Cbp6 are still necessary for Cytb hemylation in the D273-10b lab strain. Mitochondrial proteins (700 μg) from the indicated mutants were treated with acidic acetone (1% HCl), clarified, and then separated by reverse-phase HPLC. A, retention time peaks of heme b (peak b) and heme a (peak a) are represented by arbitrary units (UA). B, the area under the peak (AUP)/mg protein was calculated by the mean of two biological and one technical replicate (n = 3). Confidence interval (95%) is represented by error bars. *, p < 0.0001 versus WT.