Figure 4.

High ST6Gal-I expression provides a growth advantage. A, MiaPaCa-2 parental cells were cultured in hypoxia for 6 weeks to develop a hypoxia-adapted population. The original parental (Par) and hypoxia-adapted populations were immunoblotted for ST6Gal-I. B, MiaPaCa-2 EV and KD cells were seeded at equal density and allowed to adhere overnight in standard normoxic conditions. Cultures were then placed in hypoxia for 24, 48, or 72 h, and cell number was quantified by crystal violet staining. Values for hypoxic cultures were normalized to the overnight normoxic cultures. Values represent means and S.D. for three independent experiments. *, p < 0.05. C, representative images are shown for MiaPaCa-2 EV and KD cells grown in hypoxia for 72 h.