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ST6Gal activity increases HIF-1α mRNA levels. A and B, OV4 (A) and MiaPaCa-2 (B) cells were cultured in normoxia or hypoxia for 12 or 24 h, and then HIF-1α mRNA expression was analyzed by RT-qPCR. C and D, levels of HIF-2α mRNA (C) or Oct4 mRNA (D) were evaluated by RT-qPCR in OV4 EV and OE cells grown in normoxia or hypoxia for 12 or 24 h. Graphs depict means and S.D. from at least three independent experiments. *, p < 0.05.
