Figure 4.

Effects of TCA cycle gene mutations on V. cholerae antibiotic tolerance. Bacterial viabilities under various Tc concentrations were measured. Each mutation indicated below in different colors was introduced in N16961 (A and C) and the (p)ppGpp⁰ mutant (B and D). Bacterial strains were inoculated in LB and aerobically grown to exponential (A and B) or stationary phase (C and D). Experimental conditions were identical to those described in Fig. 1, E and H.