Table 3.
Primers used in this study
| Gene name | Directiona | Primer sequence (5′–3′)a |
|---|---|---|
| Cloning | ||
| ΔacnB left | Forward | GCAAGCATGCAAACCTCGTTTACCGTTACC |
| ΔacnB left | Reverse | CTCTGAGCTCGACTTTTTCCTCTCATTGCG |
| ΔacnB right | Forward | TTGCGAGCTCGCAGAGTGATTGAATCCTCT |
| ΔacnB right | Reverse | GCGACCCGGGATTTTGAATAAAGCTTTGCC |
| Δicd left | Forward | TATTGCATGCTGGCTTAAAGTGTCATAAGG |
| Δicd left | Reverse | GTCTAAACTAGAGAACTTTCCCTATCTGTTCT |
| Δicd right | Forward | TAGGGAAAGTTCTCTAGTTTAGACACCAAAAC |
| Δicd right | Reverse | ATGTGCATGCATACGTTCGTCCTATTGACT |
| ΔsucDC left | Forward | TATTGCATGCGTGATTTGCTCGCGATTAGC |
| ΔsucDC left | Reverse | TGGAACAACACATCTACCGCGATTACTTACTC |
| ΔsucDC right | Forward | AATCGCGGTAGATGTGTTGTTCCATTTGTTTA |
| ΔsucDC right | Reverse | ATCTGAGCTCATACCTTGAGTTTGGCGCAA |
| Δmdh left | Forward | AGTTGCATGCGCGATACTTTGGATTGGTTG |
| Δmdh left | Reverse | ATCGATTGTCGACGTAAATCTCCTTGAGAGTA |
| Δmdh right | Forward | AGGAGATTTACGTCGACAATCGATTCAAGCAT |
| Δmdh right | Reverse | ATTAGAGCTCTGAACCAATCACTAGCGCCG |
| ΔfbpA left | Forward | TGCTGCATGCTTTTAGTGTGTAAAACCACT |
| ΔfbpA left | Reverse | CGGCGAGCTCTGTATTATAGGAATGTTCAA |
| ΔfbpA right | Forward | TCGCGAGCTCGTAAAATCAGGGGTATAACG |
| ΔfbpA right | Reverse | CGCGCCCGGGATACACATAAGGATAAAGTA |
| Arbitrary PCR | ||
| KGL3-Mar1, 1 round | Forward | GGGAATCATTTGAAGGTTGGT |
| Arb1, 1 round | Reverse | GGCCACGCGTCGACTAGTACNNNNNNNNNNGATAT |
| Arb6, 1 round | Reverse | GGCCACGCGTCGACTAGTACNNNNNNNNNNACGCC |
| KGL3-Mar2, 2 round | Forward | TAGCGACGCCATCTATGTGTC |
| Arb2, 2 round | Reverse | GGCCACGCGTCGACTAGTAC |
a Restriction enzyme recognition sequences are underlined.