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. 2018 Apr 9;9:326. doi: 10.3389/fphar.2018.00326

FIGURE 4.

FIGURE 4

LC53 exerted anti-inflammatory and anti-apoptosis properties during EIU. Phosphorylation of p65 (A), HSP90 (B), Bcl-2 (C), Bax (D), and cleaved-caspase-3 (E) in eye homogenates were analyzed using Western blot. (F) Eye homogenates were examined for the protein oxidation using Oxyblot. Primary antibody specific to DNP-moiety was used to target the oxidatively modified proteins in Western blot. The specific marker (21 kDa) was shown on the left side, which represented carbonylated-trypsin inhibitor from the mixture of standard proteins. The quantification values were presented as the mean ± SEM of results from 5 to 7 animals. Representative bands from 2 to 3 independent experiments were shown for each group: the control groups (C1, C2), vehicle-treated EIU groups (V1, V2, V3), and LC53-treated EIU groups (D1, D2, D3). C, CTRL: control; V: vehicle.#p < 0.05, ##p < 0.01, ###p < 0.001 compared with the control groups treated with normal saline; ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001 compared with the EIU group treated with vehicle.