Gingerol induced apoptosis in RB355 human retinoblastoma cells after the cells were treated with 0 (A), 25 (B), 75 (C), and 150 μM (D) dose of gingerol for 48 h. The cells were then stained with Hoechst 33258 and finally analyzed by fluorescence microscopy at ×200 magnification. Orange arrows indicate cells undergoing apoptotic morphological changes, including chromatin condensation, cell shrinkage, and membrane blebbing.