Figure 5.

Gingerol induced apoptosis in RB355 human retinoblastoma cells after the cells were treated with 0 (A), 25 (B), 75 (C), and 150 μM (D) dose of gingerol for 48 h. The cells were then stained with AO/PI double-staining dye and finally analyzed by fluorescence microscopy at ×200 magnification. Yellow arrows indicate cells undergoing apoptosis, viable cells emit green fluorescence, and apoptotic cells emit yellow or red fluorescence.