Figure 2.

Dose‐dependent inhibition of the LPS‐stimulated TLR4 signal in HEK‐Blue hTLR4 cells by FP7/AMP co‐administration. HEK‐Blue hTLR4 cells were pre‐treated with increasing concentrations of FP7 and FP7/AMP mixtures and stimulated with LPS (100 ng mL⁻¹) after 30 min. Data were normalized to stimulation with LPS alone. Concentration–effect data were fitted to a sigmoidal four‐parameter logistic equation to determine IC₅₀ values. Data points represent the mean percentage ± standard error of the mean (SEM) of at least three independent experiments. Table 2 summarizes the IC₅₀ values for the inhibition of LPS‐ and PHA‐stimulated TLR4 signal in HEK‐Blue hTLR4 cells.