Figure 3.

A) Dose‐dependent PHA‐ and LPS‐stimulated TLR4 activation in HEK‐Blue hTLR4 cells. HEK‐Blue hTLR4 cells were stimulated with increasing concentrations of LPS and PHA lectin and SEAP levels in the medium were quantified after 16 h. The percentages of TLR4 activation are relative to the maximal LPS response. B) HEK‐Blue hTLR4 cells were stimulated with LPS (100 ng mL⁻¹) and PHA lectin (5 μg mL⁻¹) in the absence or presence of increasing concentrations of polymyxin B. C) Dose‐dependent inhibition of PHA‐stimulated TLR4 activation by FP7 and FP7/AMP 6. Cells were treated with increasing concentrations of compounds and stimulated with PHA‐P (5 μg mL⁻¹). The results represent data normalized with the positive control (PHA‐P alone). Concentration–effect data were fitted to a sigmoidal four‐parameter logistic equation to determine IC₅₀ values and represent the mean of percentage±SEM of at least three independent experiments. The IC₅₀ values are shown in Table 1.