Table 1.

Binding properties of full‐length chemokines and peptides derived from their N‐terminal regions towards CXCR4 and ACKR3

Name	Sequence	Binding competition
		CXCR4	ACKR3
		pIC50 ± SEM	pIC50 ± SEM
CXCL12		7.89 ± 0.04	8.74 ± 0.07
CXCL121–17	KPVSLSYRCPCRFFESH	~4.00	5.39 ± 0.10
CXCL121–9	KPVSLSYRS	<3.00	5.05 ± 0.13ns
(CXCL121–9)2	(KPVSLSYRC)2	5.31 ± 0.16	5.65 ± 0.09*
CXCL121–17D	KPVSLSYRCPCRFFESH	~4.00	<4.00
CXCL122–17	‐ PVSLSYRCPCRFFESH	~4.00	5.23 ± 0.25ns
CXCL121–17/P2G	KGVSLSYRCPCRFFESH	<4.00	4.22 ± 1.19ns
vCCL2		7.70 ± 0.06	7.47 ± 0.07
vCCL21–21	LGASWHRPDKCCLGYQKRPLP	5.71 ± 0.24	5.77 ± 0.13
vCCL21–11	LGASWHRPDKS	~4.00	5.27 ± 0.24ns
(vCCL21–11)2	(LGASWHRPDKC)2	6.67 ± 0.06	5.59 ± 0.13ns
vCCL21–21 D	LGASWHRPDKCCLGYQKRPLP	6.21 ± 0.21ns	~4.00
CXCL11		<6.00	8.45 ± 0.03
CXCL111–17	FPMFKRGRCLCIGPGVK	<4.52a	5.46 ± 0.12a
CXCL111–9	FPMFKRGRS	ND	5.09 ± 0.13ns
(CXCL111–9)2	(FPMFKRGRC)2	ND	6.03 ± 0.14*
CXCL111–17D	FPMFKRGRCLCIGPGVK	ND	5.06 ± 0.12*, a
CXCL112–17	‐ PMFKRGRCLCIGPGVK	ND	<4.52a
CXCL111–17/P2G	FGMFKRGRCLCIGPGVK	ND	6.72 ± 0.09*, a
CXCL10		<6.00	<6.00
CXCL101–17	VPLSRTVRCTCISISNQ	<4.52a	<4.52*, a

Binding competition studies with fluorescently labelled CXCL12 were performed in U87 cells (n = 5). Peptides 1‐17 (for CXCL12 and CXCL11) and 1‐21 (for vCCL2) were considered as the standard, reference peptides and monomeric mutant peptides were compared with these. Where dimeric peptides were assessed, these were compared with their corresponding monomeric peptides.

^* P < 0.05, significantly different from standard peptides or from monomeric peptides; ns, not significant, P > 0.05.

^a Highest concentration tested 30 μM. ND: not determined.