. 2018 Mar 23;175(9):1419–1438. doi: 10.1111/bph.14132

Table 4.

Binding and signalling properties of CXCL11 variants towards CXCR3 and ACKR3

Name	Sequence	Binding competition		cAMP		Calcium		Arrestin recruitment
		CXCR3	ACKR3	CXCR3		CXCR3		CXCR3		ACKR3
		pIC₅₀ ± SEM	pIC₅₀ ± SEM	pEC₅₀ ± SEM	Max (%)	pEC₅₀ ± SEM	Max (%)	pEC₅₀ ± SEM	Max (%)	pEC₅₀ ± SEM	Max (%)
CXCL11 WT	1‐FPMFKRGRCLCIGPGVKAVK‐73	8.48 ± 0.09	6.52 ± 0.05	8.21 ± 0.11	100	7.80 ± 0.07	100	7.57 ± 0.07	100	7.46 ± 0.09	100
CXCL11_Nloop10	1‐FPMFKRGRCLCISISNQAVK‐73	7.41 ± 0.11*	6.11 ± 0.07*	7.93 ± 0.13 ^ns	81	7.98 ± 0.11^ns	92	7.42 ± 0.08^ns	89	7.68 ± 0.10 ^ns	85
CXCL11_Nloop12	1‐FPMFKRGRCLCRFFESHAVK‐73	6.91 ± 0.08*	6.29 ± 0.04*	6.85 ± 0.14*	102	7.02 ± 0.06*	79	<6.00	41	7.32 ± 0.09^ns	84
CXCL11_3–73	3‐MFKRGRCLCIGPGVKAVK‐73	6.90 ± 0.09*	5.78 ± 0.07*	8.39 ± 0.21^ns	54	<6.00	37	<6.00	22	7.15 ± 0.08*	81
CXCL11_5–73	5‐KRGRCLCIGPGVKAVK‐73	6.18 ± 0.05*	5.29 ± 0.01*	<6.00		<6.00		<6.00	16	<6.00	30
CXCL11_7–73	7‐RCLCIGPGVKAVK‐73	<5.00	<5.00	<6.00		<6.00		<6.00	15	<6.00	22
CXCL11_P2G	1‐FGMFKRGRCLCIGPGVKAVK‐73	9.63 ± 0.09*	8.76 ± 0.05*	7.86 ± 0.15^ns	100	7.40 ± 0.04*	83	7.22 ± 0.14^ns	61	7.99 ± 0.07*	136

Binding competition studies with radiolabeled CXCL11 (CXCR3) or CXCL12 (ACKR3) were performed in HEK293T cells (n = 5). β‐arrestin‐2 recruitment was monitored by BRET in HEK293T cells expressing YFP‐fused receptors and Rluc‐fused β‐arrestin‐2.

P < 0.05, significantly different from WT peptide; ns. not significant.