Figure 2.

Hindlimb clasping in MLC mice. (A) Representative pictures illustrating the hindlimb clasping phenotype. Scoring was as follows: 0: normal (hindlimbs fully spread and moving); 1: intermediate (intermittent clasping of one hindlimb); 2: full (both hindlimbs fully drawn up to the abdomen). Left: wildtype, scored 0; middle, right: Mlc1‐null and Glialcam‐null mice respectively, both scored 2. (B) Both Mlc1‐null (n = 12) and Glialcam‐null (n = 8) mice exhibited significantly increased hindlimb clasping severity compared to the wild‐type controls (n = 14; p = 0.0003; wild‐type vs Mlc1‐null, p = 0.0003; wild‐type vs Glialcam‐null, p = 0.014). Open circles show individual mice. Bars indicate mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001. MLC = megalencephalic leukoencephalopathy with subcortical cysts. [Color figure can be viewed at http://www.annalsofneurology.org]