Figure 1.

PHYA mRNA abundance increases in tomato fruit during ripening. Fruit (cv UC-82B) at five different developmental stages were harvested on the same day from plants in the greenhouse. mRNA was isolated from these fruit, separated electrophoretically, and blotted to nylon. RNA blots were subsequently hybridized with a ³²P-labeled probe derived from a conserved region of PHYA, washed at high stringency, and exposed to a phosphor plate. Images were developed using a phosphor imager (Molecular Dynamics, Sunnyvale, CA). The lower panel shows the absolute abundance of PHYA mRNA. IG, Immature-green; MG, mature-green; B, breaker; T, turning/orange; RR, red-ripe.