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. 2018 Jan 16;26(2):1670–1680. doi: 10.1364/OE.26.001670

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© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

© 2018 Optical Society of America under the terms of the OSA Open Access Publishing Agreement

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Fig. 7 — Super-resolution images of fixed neurons. Neurons. (a) PALM image of actin-mEos3.2 of fixed neurons before and after correction. The insets show the 3D visualization of the spines inside of the white rectangles (see Visualization 1^{(12.4MB, avi)}). (b) The position of the fiduciary marker used for the image in 3D. The drift along z axis is relatively smaller (100 nm) than that of lateral dimension (~500 nm, x and y). (c) The linear profile of the selected area shows the width of the spine before and after the drift correction as FWHM of the Gaussian fit.