Table III.
Pool sizes of the intermediates in Cho synthesis used to model 33P-EA, 33P-MME, and [14C]formate labeling experiments
| Metabolitea |
33P-MME
|
33P-EA
|
[14C]Formate
|
Meana (se) | ||
|---|---|---|---|---|---|---|
| Expt. 1 | Expt. 2 | Expt. 1 | Expt. 1 | Expt. 2 | ||
| nmol g−1 fresh wt | ||||||
| P-EA | – | – | 15 | 6 | 6 | 9 (3) |
| P-EA (storage) | – | – | 100 | 100 | 100 | 100 (0) |
| P-MME | 10 | 10 | 2 | 3 | 1.5 | 5.3 (1.9) |
| P-MME (storage) | 5 | 5 | 10 | 5 | 5 | 6 (1) |
| P-DME | 12 | 5 | 0.3 | 1 | 1 | 3.9 (2.2) |
| P-Cho | 100b | 40 | 30 | 30 | 40 | 48 (13) |
| Ptd-EA | – | – | 750 | 750 | 750 | 750 (0) |
| Ptd-MME | 4 | 6 | 2.5 | 3 | 5 | 4.1 (0.6) |
| Ptd-DME | 1 | 3 | 2 | 2 | 3 | 2.2 (0.4) |
| Ptd-Cho | 1,600 | 1,800 | 1,500 | 1,500 | 1,500 | 1,580 (58) |
| Cho | – | – | – | 2 | 2 | 2 (0) |
| Cho (storage) | – | – | – | 70 | 70 | 70 (0) |
| Phosphatidic acid | 100 | 100 | 100 | – | – | 100 (0) |
| Pi | 10,000 | 10,000 | 10,000 | – | – | 10,000 (0) |
a
Mean of model output values from all the labeling experiments.
b
Values in italics are based directly on the range of measured values; nonitalicized values are model-derived estimates.