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. 2016 Aug 24;112(2):532–542. doi: 10.1093/cvr/cvw200

Figure 2.

Figure 2

Expression of Sema6D in AV cushion mesenchymal cells is upregulated by BMP4 stimulation. ( A , B ) tsA58-AVM cells were treated with BMP4 (100 ng/ml) for 24 h followed by Northern ( A ) and Western analysis ( B ). GAPDH and Actin were used as loading controls. ( C ) AV cushion mesenchymal cells at E10.5 were treated with BSA, BMP4 (100 ng/ml) or NOGGIN (100 ng/ml) for 24 h followed by qRT-PCR analysis to detect Sema6D . Hprt was included as the loading control. The Sema6D expression level in cells treated with BSA was set at 100%. Data were averaged from six independent cultures with error bars indicating SEM. ** P <  0.01, Student’s t -test.