Number of CD86-positive cells in atrial tissue. (A) Representative
figures of an immunohistological staining of atrial sections by using an
anti-CD86-specific antibody. (B) Quantification of CD86-positive
cells in atrial tissue reveal a significant higher amount of these cells in AngII
(+)/OA-NO2 (−) animals (n = 5/4/4). Furthermore, this
was not significantly influenced by a parallel treatment with OA-NO2. For
the staining of paraffin-embedded murine atrial sections with CD86 (1 : 500, rabbit
IgG, NB110–55488, Novus Biologicals, USA), a standard protocol was used. For
quantification, CD86-positive cells were counted in atrial sections. Scale bar = 50
µm. ***P < 0.001.