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. 2018 Mar 23;175(9):1486–1503. doi: 10.1111/bph.14156

Figure 2.

Figure 2

Co‐treatment of metformin and T317 maintains structural integrity of aortic wall and inhibits calcium deposits in aortic lesion areas. The aortic root cross sections were assessed by the following assays: (A–C) Haematoxylin and eosin staining (A) followed by quantitative analysis of necrotic core area (B) and fibrous cap area (C). nc: necrotic cores marked by a black dashed line; fc: fibrous cap marked by a blue dashed line; (D, E) VVG staining (D) followed by quantitative analysis of collagen content (E); (F, G) immunofluorescent staining for expression of SMA (F) with quantification of SMA positive areas (G); (H, I) Alizarin Red S staining for calcification (H, indicated by black arrows) and quantification of calcification positive areas (I). *P < 0.05, significantly different from control or as indicated (n = 15). C, control; T, T317; M, metformin; T/M, T317 plus metformin.