Figure 7.

Metformin ameliorates TG metabolism in vivo by regulating expression of genes for TG synthesis and hydrolysis, and fatty acid oxidation. Total protein, RNA and frozen sections were prepared from mouse liver samples used in Figure 1 and completed the following assays respectively: (A) expression of DGAT1, ATGL, HSL, PPARα and CAT protein was determined by immunofluorescent staining with quantification of the mean immunofluorescent intensity (MFI) of images; (B) expression of DGAT1, ATGL, HSL and PPARα protein was determined by Western blot, *P < 0.05, significantly different as indicated (n = 6); (C) expression of DGAT1, ATGL, HSL, PPARα and CAT mRNA was determined by qRT‐PCR, *P < 0.05, significantly different as indicated (n = 6).