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. 2018 Mar 1;8(7):3599–3608. doi: 10.1002/ece3.3888
Locus Primer sequence (5′→3′) Repeat motif Allele size range (bp) Ta (°C) Fluorescent dye BLAST to hit description [organism]
BC1 F: TACTCCGAAACTCACGCCTT (CTT)7 270–273 59 6‐FAM Nonintrinsic ABC protein 6, putative isoform 2
[Theobroma cacao]
R: AAAGACGTATCGGTGAAGCG
BC5 F: ACAAAAATGGCGTCTTCTGG (CAG)7 128–134 60 6‐FAM
R: GCAGGAGATCCATGGTGATT
BC9 F: TTTGAAAGGGAGGGTGTTTG (GACT)6 134–138 57 HEX
R: GAGGAGGAAAAGTTATGTTTTGG
BC10 F: ACCTCCTGCACAGACCATTC (ACA)8 213–216 60 6‐FAM
R: CATGGGGGAAAATTTTGTTG
BC11 F: TGGGAGCTGAGATTTGATCC (CAGC)6 316–320 60 NED
R: CCCCACTGGATTGATTGATT
BC12 F: TCCATCCAAATTCAGCAACA (CAG)8 147–150 60 HEX Auxin efflux facilitator isoform 6
[Theobroma cacao]
R: GGTTTGCTGCAAGGAGAGTC